IMP-10457

Il4

Recombinant Rat IL-4 protein (Cys25-Ser147) with an N-terminal Met was expressed in E. coli.

The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

E. coli

Rat

Tag Free

Met

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Predicted Molecular Mass: 13.5 kDa

Cys25-Ser147

Measured in a cell proliferation assay using rat splenocytes. Noble, A. and D.M. Kemery (1995) Immunology 85:357. The ED50 for this effect is 0.025-0.25 ng/mL.

<0.01 EU/μg of the protein by the LAL method.

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.