Recombinant Rat Il6 Protein (25-211aa), C-His-Tagged

Product Description

Cat

IMP-9474

Official Symbol

Il6

Product Overview

Recombinant rat Il6, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Description

Il6, also known as interleukin 6, is an alpha-helical phosphorylated and glycosylated cytokine. This protein is important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism and cancer progression. Also, it can function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Expression System

Baculovirus

Species

Rat

Tag

C-His

Form

Liquid in Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Molecular Mass

22.8 kDa (196 aa)

Protein length

25-211

AA Sequence

<ADP>FPTSQVRRGDFTEDTTHNRPVYTTSQVGGLITYVLREILEMRKELCNGNSDCMNSDDALSENNLKLPEIQRNDGCFQTGYNQEICLLKICSGLLEFRFYLEFVKNNLQDNKKDKARVIQSNTETLVHIFKQEIKDSYKIVLPTPTSNALLMEKLESQKEWLRTKTIQLILKALEEFLKVTMRSTRQT<HHHHHH>

Endotoxin

< 1 EU/μg of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Applications

SDS-PAGE,Bioactivity

Notes

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Storage

Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Concentration

0.5 mg/mL (determined by absorbance at 280nm)

SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤0.1 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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