Recombinant Rat Il6 Protein (25-211aa), C-His-Tagged

Product Description

Cat

IMP-9474

Official Symbol

Il6

Product Overview

Recombinant rat Il6, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Description

Il6, also known as interleukin 6, is an alpha-helical phosphorylated and glycosylated cytokine. This protein is important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism and cancer progression. Also, it can function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Expression System

Baculovirus

Species

Rat

Tag

C-His

Form

Liquid in Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Molecular Mass

22.8 kDa (196 aa)

Protein length

25-211

AA Sequence

<ADP>FPTSQVRRGDFTEDTTHNRPVYTTSQVGGLITYVLREILEMRKELCNGNSDCMNSDDALSENNLKLPEIQRNDGCFQTGYNQEICLLKICSGLLEFRFYLEFVKNNLQDNKKDKARVIQSNTETLVHIFKQEIKDSYKIVLPTPTSNALLMEKLESQKEWLRTKTIQLILKALEEFLKVTMRSTRQT<HHHHHH>

Endotoxin

< 1 EU/μg of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Applications

SDS-PAGE,Bioactivity

Notes

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Storage

Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Concentration

0.5 mg/mL (determined by absorbance at 280nm)

SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤0.1 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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