Recombinant Rat JAG1 Protein (Ser32-Asp1067), C-6×His-tagged

Product Description

Cat

IMP-9994

Official Symbol

JAG1

Product Overview

Recombinant Rat Jagged 1 protein (Ser32-Asp1067) with substitutions GGARN56-60AE and DR63-64TLVRPY and a C-terminal 6×His tag was expressed in Mouse myeloma cell line.

Description

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter is involved in signaling processes. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.

Expression System

Mouse myeloma cell line

Species

Rat

Tag

C-6×His

Predicted N Terminal

Ser32

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Molecular Mass

Predicted Molecular Mass: 114 kDa
SDS-PAGE: 134-160 kDa, reducing conditions

Protein length

Ser32-Asp1067

Bio-activity

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.4-2.4 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

12 months from date of receipt, ≤ -20 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.4-2.4 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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