Recombinant Rat Nrp1 Protein (Phe22-Asp854, Lys811Arg, Pro812-Gly828 del), C-Fc and 6×His-tagged

Product Description

Cat

IMP-9838

Official Symbol

Nrp1

Product Overview

Recombinant Rat Neuropilin-1 protein, Phe22-Asp854 (Lys811Arg and Pro812-Gly828 del), with a human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Spodoptera frugiperda, Sf 21 (baculovirus) cells.

Description

Enables semaphorin receptor activity. Involved in negative regulation of axon extension involved in axon guidance and response to wounding. Located in cell surface; growth cone; and neuronal cell body. Human ortholog(s) of this gene implicated in lung non-small cell carcinoma. Orthologous to human NRP1 (neuropilin 1).

Expression System

Sf 21 cells

Species

Rat

Tag

C-Fc and 6×His

Predicted N Terminal

Phe22

Form

Lyophilized from a 0.2 μm filtered solution in Citrate Phosphate and NaCl.

Molecular Mass

Predicted Molecular Mass: 119 kDa (monomer)
SDS-PAGE: 130-150 kDa, reducing conditions

Protein length

Phe22-Asp854, Lys811Arg, Pro812-Gly828 del

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized rrNeuropilin-1/Fc Chimera can bind rhVEGF165 with an apparent KD < 1 nM.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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