Recombinant Rat NT5E protein, C-His tag

Product Description

Cat

IMP-685

Official Symbol

NT5E

Product Overview

Recombinant Rat NT5E(P21588)(Trp29-Lys549) protein, fused with a 6*His tag at the C-terminus, was expressed from HEK293 Cells.

Expression System

HEK293

Species

Rat

Tag

C-His tag

Form

Liquid in sterile 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.

Molecular Mass

The protein has a calculated MW of 59kDa, and migrates as 70-90kDa under reducing conditions.

Protein length

Trp29-Lys549

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Concentration

Please refer to the COA for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Rat CD73-His at 2μg/ml (100 μl/well) can bind Anti-Human CD73 mAb. The ED50 of Anti-Human CD73 mAb is 8.55 ng/ml.

Bioactivity-BLI 1

Loaded Anti-Human CD73 mAb-Fc on Protein A Biosensor, can bind Rat CD73-His with an affinity constant of 0.20 nM as determined in BLI assay.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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