Recombinant Rat PVR Protein (Glu34-Leu351), C-6×His-tagged
Product Description
Cat
IMP-9987
Official Symbol
PVR
Product Overview
Recombinant Rat CD155/PVR protein (Glu34-Leu351) with a C-terminal 6×His tag was expressed in Human embryonic kidney cell.
Description
The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.
Expression System
HEK293
Species
Rat
Tag
C-6×His
Predicted N Terminal
Glu34
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 35 kDa
SDS-PAGE: 62-71 kDa, reducing conditions
Protein length
Glu34-Leu351
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Rat CD155/PVR is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD96 that produces 50% optimal binding response is 1.5-7.5 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Rat CD155/PVR is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD96 that produces 50% optimal binding response is 1.5-7.5 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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