Recombinant Rat PVR Protein (Glu34-Leu351), C-mFc-tagged
Product Description
Cat
IMP-9985
Official Symbol
PVR
Product Overview
Recombinant Rat CD155/PVR protein (Glu34-Leu351) with a Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Human embryonic kidney cell.
Description
The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.
Expression System
HEK293
Species
Rat
Tag
C-mFc
Predicted N Terminal
Glu34
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 61 kDa
SDS-PAGE: 92-108 kDa, reducing conditions
Protein length
Glu34-Leu351
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD96 is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Rat CD155/PVR Fc Chimera that produces 50% optimal binding response is 1-8 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD96 is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Rat CD155/PVR Fc Chimera that produces 50% optimal binding response is 1-8 ng/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info
Phone
  • 1-631-559-9269
    1-516-512-3133
Fax
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