Recombinant Rat TIGIT protein, C-Fc Tag

Product Description

Cat

IMP-187

Official Symbol

TIGIT

Product Overview

Recombinant Rat TIGIT protein(AA Ala 17-Ala 138)(Accession # D3ZTQ2) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Rat

Tag

C-Fc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 39.9 kDa. As a result of glycosylation, the protein migrates as 48-55 kDa under reducing (R) condition, and 85-110 kDa under non-reducing (NR) condition (SDS-PA

Protein length

Ala 17-Ala 138

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Rat TIGIT, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human Nectin-2, His Tag at 5 ug/mL (100 uL/well) can bind Rat TIGIT, Fc Tag with a linear range of 0.039-5 ug/mL.

Bioactivity-BLI 1

Loaded Rat TIGIT, Fc Tag on Protein A Biosensor, can bind Human Nectin-2, His Tag with an affinity constant of 0.59 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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