Recombinant Rat TNFa protein, N-His Tag
Product Description
Cat
IMP-2295
Official Symbol
TNFa
Product Overview
Recombinant Rat TNFa protein(P16599)(Leu80~Leu235), fused with N-terminal His Tag, was expressed in E. coli.
Expression System
E. coli
Species
Rat
Tag
N-His Tag
Form
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Molecular Mass
53kDa
Protein length
Leu29~Thr500
Purity
> 90%
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Western Blot 1
Western Blot

SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
The concentration of IL-1β in the cell supernatant of A549 cells stimulated by TNF-α.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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