Recombinant Rat TNFRSF4 Protein (Val20-Pro210), C-mFc-tagged
Product Description
Cat
IMP-9923
Official Symbol
Tnfrsf4
Product Overview
Recombinant Rat OX40/TNFRSF4 protein (Val20-Pro210) with a Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation.
Expression System
Mouse myeloma cell line
Species
Rat
Tag
C-mFc
Predicted N Terminal
Val20&Val22
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 48 kDa (monomer)
SDS-PAGE: 67-78 kDa reducing conditions
Protein length
Val20-Pro210
Bio-activity
Measured by its binding ability in a functional ELISA. When recombinant rat OX40 Ligand is coated at 75 ng/mL (100 μL/well), the concentration of Recombinant OX40 Fc Chimera that produces 50% optimal binding response is 1-5 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE with silver staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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