Recombinant Rat TNFRSF4 Protein (Val20-Pro210), C-mFc-tagged
Product Description
Cat
IMP-9923
Official Symbol
Tnfrsf4
Product Overview
Recombinant Rat OX40/TNFRSF4 protein (Val20-Pro210) with a Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation.
Expression System
Mouse myeloma cell line
Species
Rat
Tag
C-mFc
Predicted N Terminal
Val20&Val22
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 48 kDa (monomer)
SDS-PAGE: 67-78 kDa reducing conditions
Protein length
Val20-Pro210
Bio-activity
Measured by its binding ability in a functional ELISA. When recombinant rat OX40 Ligand is coated at 75 ng/mL (100 μL/well), the concentration of Recombinant OX40 Fc Chimera that produces 50% optimal binding response is 1-5 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE with silver staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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