Recombinant Rat Tnfsf11 Protein (Arg72-Asp318), N-6×His-tagged
Product Description
Cat
IMP-9960
Official Symbol
Tnfsf11
Product Overview
Recombinant Rat TRANCE/RANK L/TNFSF11 protein (Arg72-Asp318), with an N-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
Predicted to enable cytokine activity; identical protein binding activity; and tumor necrosis factor receptor superfamily binding activity. Involved in several processes, including positive regulation of bone resorption; positive regulation of osteoclast differentiation; and response to immobilization stress. Predicted to be located in extracellular space and plasma membrane. Predicted to be integral component of membrane. Biomarker of mucopolysaccharidosis VI and myocarditis. Human ortholog(s) of this gene implicated in autosomal recessive osteopetrosis 2 and osteoporosis. Orthologous to human TNFSF11 (TNF superfamily member 11).
Expression System
CHO
Species
Rat
Tag
N-6×His
Predicted N Terminal
His
Form
Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 29 kDa
SDS-PAGE: 33-38 kDa, reducing conditions
Protein length
Arg72-Asp318
Bio-activity
Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 2.5-15 ng/mL in the presence of 2.5 μg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 2.5-15 ng/mL in the presence of 2.5 μg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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