Recombinant Rat TNFSF18 Protein (Thr74-Ser200), N-HA-tagged

Product Description

Cat

IMP-9984

Official Symbol

Tnfsf18

Product Overview

Recombinant Rat GITR Ligand/TNFSF18 protein (Thr74-Ser200) with a N-terminal HA-tag was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells.

Expression System

Mouse myeloma cell line

Species

Rat

Tag

N-HA

Predicted N Terminal

Tyr

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 16 kDa
SDS-PAGE: 19-22 kDa, reducing conditions

Protein length

Thr74-Ser200

Bio-activity

Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with rat GITR. The ED50 for this effect is 0.06-0.6 μg/mL in the presence of Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

12 months from date of receipt, ≤ -20 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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