Recombinant Rat VEGFA protein, N-His Tag

Product Description

Cat

IMP-2309

Official Symbol

VEGFA

Product Overview

Recombinant Rat VEGFA protein(P16612)(Ala27~Arg190), fused with N-terminal His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Rat

Tag

N-His Tag

Form

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Molecular Mass

50kDa

Protein length

Ala298~Leu441

Purity

> 97%

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Western Blot 1

SDS-PAGE

Bioactivity-ELISA 1

Cell proliferation of ECV304 cells after stimulated with VEGFA

(A)ECV304 cells cultured in serun-free DMEM,stimulated with 10ng/ml VEGFA for 72h; (B)Ustimulated ECV304 cells cultured in serum-free DMEM for 72h.

Bioactivity-ELISA 2

The dose-effect curve of VEGFA on ECV304 cells

The dose-effect curve of VEGFA was shown in Figure 2. It was obvious that VEGFA significantly promoted cell proliferation of ECV304 cells. The ED50 for this effect is typically 5.58 to 9.98 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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