Recombinant Rhesus CD3D&CD3E Protein, No Tag

Product Description

Cat

IMP-4236

Official Symbol

CD3D&CD3E

Product Overview

Recombinant rhesus CD3D extracellular domain (F6WI60)(Met1-Ala105) with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant rhesus CD3E extracellular domain(XP_014971302.1) (Met1-Asp117) was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the CD3D&CD3E heterodimer was purified.

Description

The CD3 complex mediates signal transduction.

Expression System

HEK293

Species

Rhesus

Tag

No Tag

Predicted N Terminal

Gln 22&Phe 22

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant heterodimer of rhesus CD3D&CD3E comprises 679 (346+333) amino acids and has a calculated molecular mass of 76.7 (39.1+ 37.6) KDa. The apparent molecular mass of rhesus CD3D&CD3E heterodimer is approximately 52&43 KDa respectively in SDS-PAGE.

Protein length

Met1-Ala105 and Met1-Asp117

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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