Recombinant Rhesus CD3E&CD3G Protein, C-hFc-tagged

Product Description

Cat

IMP-4243

Official Symbol

CD3E&CD3G

Product Overview

Recombinant rhesus CD3E (XP_014971302.1) (Met1-Asp117) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant rhesus CD3G (NP_001253854.1) (Met1-Thr113) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the cynomolgus CD3E/CD3G heterodimer was purified.

Description

The CD3 complex mediates signal transduction.

Expression System

HEK293

Species

Rhesus

Tag

C-hFc

Predicted N Terminal

Gln 22&Gln 23

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant rhesus CD3E/CD3G consists of 669 (337+332) amino acids and has a calculated molecular mass of 75.4 (37.89+37.52) KDa.

Protein length

Met1-Asp117 and Met1-Thr113

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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