Recombinant rhesus CD3E (XP_014971302.1) (Met1-Asp117) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant rhesus CD3G (NP_001253854.1) (Met1-Thr113) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the cynomolgus CD3E/CD3G heterodimer was purified.
Description
The CD3 complex mediates signal transduction.
Expression System
HEK293
Species
Rhesus
Tag
C-hFc
Predicted N Terminal
Gln 22&Gln 23
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant rhesus CD3E/CD3G consists of 669 (337+332) amino acids and has a calculated molecular mass of 75.4 (37.89+37.52) KDa.
Protein length
Met1-Asp117 and Met1-Thr113
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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