Recombinant Rhesus ERBB3 Protein, C-hFc-tagged
Product Description
Cat
IMP-4559
Official Symbol
ERBB3
Product Overview
Recombinant Rhesus (Macaca mulatta) ErbB3 (XP_001113953.2) extracellular domain (Met 1-Thr 643) was fused with the Fc region of human IgG1 at the C-terminus.
Description
ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
Expression System
HEK293
Species
Rhesus
Tag
C-hFc
Predicted N Terminal
Ser 20
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant Rhesus ErbB3/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 865 amino acids and predicts a molecular mass of 95.7 kDa. As a result of glycosylation, rhesus ErbB3/Fc monomer migrates as an approximately 130-140 kDa band in SDS-PAGE under reducing conditions.
Protein length
Met1-Thr643
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 90 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Measured by its binding ability in a functional ELISA. Immobilized human NRG1 Alpha His at 2 μg/mL (100 μL/well) can bind Rhesus HER3/ERBB3 hFc, the EC50 of Rhesus HER3/ERBB3 hFc is 250-800 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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