Recombinant Rhesus FCGR3 Protein, C-His&C-AVI-tagged

Product Description

Cat

IMP-1904

Official Symbol

FCGR3

Product Overview

Recombinant rhesus CD16 (NP_001258584.1) extracellular domain (Met 1-Gln 208) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.

Description

Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.

Expression System

HEK293

Species

Rhesus

Tag

C-His&C-AVI

Predicted N Terminal

Gly 17

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant rhesus CD16 consists of 223 amino acids and has a calculated molecular mass of 25.3 kDa.

Protein length

Met1-Gln208

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-BLI 1

Labeled biotin to CD16 / FCGR3 Protein, Rhesus, Recombinant (His&AVI Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of CD16 / FCGR3 Protein, Rhesus, Recombinant (His&AVI Tag), Biotinylated bound to IgG1 Antibody was 0.1 μM.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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