Recombinant Rhesus macaque CD40 protein, C-Fc Tag

Product Description

Cat

IMP-195

Official Symbol

CD40

Product Overview

Recombinant Rhesus macaque CD40 protein(AA Glu 21-Arg 193)(Accession # NP_001252791.1) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Rhesus macaque

Tag

C-Fc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 45.9 kDa. The protein migrates as 56 kDa under reducing (R) condition (SDS-PAGE).

Protein length

Glu 21-Arg 193

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Rhesus macaque CD40, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Rhesus macaque CD40, Fc Tag at 2 ug/mL (100 uL/well) can bind Human CD40 Ligand, His Tag with a linear range of 16-63 ng/mL.

Bioactivity-BLI 1

Loaded Rhesus macaque CD40, Fc Tag on Protein A Biosensor, can bind Human CD40 Ligand, His,Flag Tag (active trimer) (MALS verified) with an affinity constant of 0.829 nM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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