Recombinant Rhesus macaque PVR protein, C-His Tag

Product Description

Cat

IMP-127

Official Symbol

PVR

Product Overview

Recombinant Rhesus macaque PVR protein(AA Asp 28-Asn 343)(Accession # Q0MSE6-1) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Rhesus macaque

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 36.3 kDa. The protein migrates as 48-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Asp 28-Asn 343

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Rhesus macaque CD155, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA 1

Immobilized Rhesus macaque CD155, His Tag at 5 ug/mL (100 uL/well) can bind Cynomolgus / Rhesus macaque TIGIT, Fc Tag with a linear range of 0.02-0.313 ug/mL.

Bioactivity-ELISA 2

Immobilized Rhesus macaque CD155, His Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human DNAM-1, Fc,Avitag with a linear range of 10-156 ng/mL.

Bioactivity-BLI 1

Loaded Human DNAM-1, Fc Tag on Protein A Biosensor, can bind Rhesus macaque CD155, His Tag with an affinity constant of 0.47 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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