Recombinant Rhesus TNF protein
Product Description
Cat
IMP-5890
Official Symbol
TNF
Product Overview
Recombinant Rhesus TNF protein(P48094)(77-233aa) was expressed in E.Coli.
Expression System
E.Coli
Species
Rhesus
Tag
N/A
Form
Lyophilized from a 0.2 µm filtered PBS, pH 7.4, 5 % trehalose
Molecular Mass
17.3 kDa
Protein length
77-233aa
AA Sequence
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL
Endotoxin
Less than 1.0 EU/µg as determined by LAL method.
Purity
>95% as determined by SDS-PAGE and HPLC.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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