Recombinant Rhesus TNF protein
Product Description
Cat
IMP-5890
Official Symbol
TNF
Product Overview
Recombinant Rhesus TNF protein(P48094)(77-233aa) was expressed in E.Coli.
Expression System
E.Coli
Species
Rhesus
Tag
N/A
Form
Lyophilized from a 0.2 µm filtered PBS, pH 7.4, 5 % trehalose
Molecular Mass
17.3 kDa
Protein length
77-233aa
AA Sequence
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL
Endotoxin
Less than 1.0 EU/µg as determined by LAL method.
Purity
>95% as determined by SDS-PAGE and HPLC.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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