Recombinant SARS-CoV-2 NSP8 Protein (Ala1-Gln198), C-8×His-tagged

Product Description

Cat

IMP-9612

Official Symbol

NSP8

Product Overview

Recombinant SARS-CoV-2 NSP8 protein (Ala1-Gln198) with a C-terminal 3C protease cleavage site and 8-His tag was expressed in E. coli.

Description

Non-structural protein 8 (NSP8) is one of several functional proteins released by ORF1a-encoded protease cleavage of the pp1a and pp1ab replicase polyproteins expressed from the coronavirus (CoV) genome. The NSPs are involved in the replication and transcription of the viral RNA and not incorporated within the virion particles. Coronaviruses include various highly pathogenic strains such as SARS-CoV, MERS-CoV and SARS-CoV2 that have had significant impact on humans as well as strains that have negatively impacted livestock. NSP8 is a small 198 amino acid protein that forms a dimer with NSP7 and subsequently assembles into a large hexadecameric structure. The NSP8 sequence is highly conserved across coronaviruses.

Expression System

E. coli

Species

SARS-CoV-2

Tag

C-8×His

Predicted N Terminal

Ala1

Form

Supplied as a 0.2 μm filtered solution in HEPES and NaCl.

Molecular Mass

Predicted Molecular Mass: 24 kDa
SDS-PAGE: 24 kDa, under reducing conditions.

Protein length

Ala1-Gln198

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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