Recombinant SARS-CoV-2 ORF1ab Protein, N-His&N-AVI-tagged

Product Description

Cat

IMP-4366

Official Symbol

ORF1ab

Product Overview

Recombinant SARS-CoV-2 (2019-nCoV) 3CLpro (YP_009725295.1) (Ser3264-Gln3569) was expressed with a N-terminus polyhistide tag and AVI tag at the N-terminus.

Description

3C-like protease (3CLpro) is the main protease of Humann Coronavirus. 3C-like protease (3CLpro) is a key enzyme, as it cleaves several sites to produce non-structural proteins that are essential for genome replication and Coronavirus virion production, such as an RNA-dependent RNA polymerase, a helicase, ribonucleases and 3CLpro itself, from two types of polyproteins (pp1a and pp1ab). SARS-CoV 3CLpro exists as a homodimer and each protomer has an active site.

Expression System

Insect cell

Species

SARS-CoV-2

Tag

N-His&N-AVI

Predicted N Terminal

Met

Form

Lyophilized from sterile 20mM Tris, 300mM NaCl, 10% glycerol, pH 8.0, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant heterodimer of SARS-CoV-2 (2019-nCoV) 3CLpro consists of 332 amino acids and has a calculated molecular mass of 37.12 KDa.

Protein length

Ser3264-Gln3569

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry