Recombinant Sus scrofa (Pig) IL10 Protein, No Tag
Product Description
Cat
IMP-4612
Official Symbol
IL10
Product Overview
Recombinant porcine IL10 (Q29055) (Ser19-Asn175) was expressed with an initial Met at the N-terminal.
Description
IL-10 is an anti-inflammatory cytokine that belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells, and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. The importance of interleukin 10 for counteracting excessive immunity in the human body is revealed by the fact that patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant IL-10. IL-10 inhibits the synthesis of some cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and by helper T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen-presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production.
Expression System
E. coli
Species
Sus scrofa (Pig)
Tag
No Tag
Predicted N Terminal
Met
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant porcine IL10 consists of 158 amino acids and has a predicted molecular mass of 18.2 kDa. The apparent molecular mass of it is approximately 18 kDa in SDS-PAGE under reducing conditions.
Protein length
Ser19-Asn175
Endotoxin
Please contact us for more information.
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Measured by its binding ability in a functional ELISA. Immobilized sus scrofa (pig) IL10 at 2 μg/mL (100 μL/well) can bind Rhesus IL10RA-hFc, the EC50 of Rhesus IL10RA-hFc is 25-100 ng/mL.

Bioactivity-Cell based assay 1
Bioactivity-Cell based assay

Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is typically 1-5 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info