Recombinant Sus scrofa (Pig) IL6 Protein, No Tag

Product Description

Cat

IMP-4750

Official Symbol

IL6

Product Overview

Recombinant sus scrofa IL6 (NP_999564.1) (Pro29-Met212) was expressed with an initial Met.

Description

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing the transition from innate to acquired immunity.

Expression System

E. coli

Species

Sus scrofa (Pig)

Tag

No Tag

Predicted N Terminal

Met

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant sus scrofa IL6 consists 185 amino acids and predicts a molecular mass of 21.2 kDa.

Protein length

Pro29-Met212

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized sus scrofa (pig) IL6 at 2 μg/mL (100 μL/well) can bind Human IL-6R-His, the EC50 of Human IL-6R-His is 250-750 ng/mL.

Bioactivity-Cell based assay 1

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 1-5 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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