Recombinant Yeast UBB Protein (Met1-Gly76), Tag Free
Product Description
Cat
IMP-10097
Official Symbol
UBB
Product Overview
Recombinant Yeast Ubiquitin protein (Met1-Gly76) without tag was expressed in E. coli.
Description
Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin. In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting.
Expression System
E. coli
Species
Yeast
Tag
Tag Free
Form
Lyophilized from a solution in deionized water.
Molecular Mass
Predicted Molecular Mass: 8.6 kDa
Protein length
Met1-Gly76
Bio-activity
Recombinant Yeast Ubiquitin can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Yeast Ubiquitin concentration of 0.2-1 mM.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 10 mg/mL in an aqueous solution.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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