ISK-024

TSLP; IL7RA&CRLF2

ID Components Size (96 tests)
ISK-024-1 High-bind Plate 1 plate
ISK-024-2 Human IL-7 R alpha & TSLP R 25 μg
ISK-024-3 Anti- TSLP Neutralizing Antibody 20 μg
ISK-024-4 Human TSLP-Biotin 10 μg
ISK-024-5 Streptavidin-HRP 10 μg
ISK-024-6 Coating Buffer 12 mL
ISK-024-7 10xWashing Buffer 50 mL
ISK-024-8 Blocking Buffer 50 mL
ISK-024-9 Substrate Solution 12 mL
ISK-024-10 Stop Solution 7 mL

Thymic stromal lymphopoietin (TSLP) exerts its biological effects by binding to a high-affinity heteromeric complex composed of thymic stromal lymphopoietin receptor chain and IL-7Rα.The formation of the ternary complex, TSLPR:TSLP: IL-7Rα,initiates signaling in cells co-expressing TSLPR and IL-7Rα.TSLP has been implicated in a variety of allergic diseases (e.g., atopic dermatitis, bronchial asthma, eosinophilic esophagitis). Therefore, inhibition of TSLP interaction with TSLP receptor complex has been considered a promising strategy to blocks thymic stromal lymphopoietin (TSLP) function and prevents the overreactive immune response to allergic,eosinophilic and other types of airway inflammation associated with severe asthma.

96 tests

8-0.0156 μg/mL

This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new TLSP pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human TLSP to immobilized human IL-7 R alpha & TSLP R in a functional ELISA assay, and employs a simple colorimetric sandwich ELISA platform.
Your experiment will include 4 simple steps:
a) Coat the plate with human IL-7 R alpha & TSLP R.
b) Add your molecule of interest to the tests.
c) Add human TLSP-Biotin to bind the coated human IL-7 R alpha & TSLP R.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit TSLP: IL-7 R alpha & TSLP Rbinding will be determined by comparing OD readings among different experimental groups.

This kit is developed for screening for inhibitors of TSLP binding to human IL-7Rα & TSLP R.

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Serial dilutions of Anti-TSLP Neutralizing antibody (1:1 serial dilution, from 8 μg/mL to 0.0156 μg/mL (55.317-0.108 nM)) was added into IL-7 R alpha & TSLP R: TSLP-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting.