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Human PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.
The purity of Human PD-L1, His Tag was more than 90% and the molecular weight of this protein is around 35-50 kDa verified by SEC-MALS.
Immobilized Human PD-L1, His Tag at 1 ug/mL (100 uL/well) can bind Anti-Human PD-L1 MAb (Human IgG1) with a linear range of 0.1-3 ng/mL.
Immobilized Human PD-L1, His Tag at series of concentration on Anti-His Tag (C-term) Antibody precoated (0.1 ug/well) plate, can bind Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) at 5 ug/mL (100 uL/well) with a linear range of 10-78 ng/mL.
Loaded Human PD-1, Fc Tag, low endotoxin on ProteinA Biosensor, can bind Human PD-L1, His Tag with an affinity constant of 5.3 uM as determined in BLI assay (ForteBio Octet Red96e).
Loaded Anti-Human PD-L1 MAb (Human IgG1) on AHC Biosensor, can bind Human PD-L1, His Tag with an affinity constant of 1.61 nM as determined in BLI assay (ForteBio Octet Red96e).
Human PD-1, Fc Tag captured on CM5 chip via anti-human IgG Fc antibody, can bind Human PD-L1, His Tag with an affinity constant of 3.6 uM as determined in a SPR assay (Biacore T200).
Anti-Human PD-L1 Mab (Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human PD-L1, His Tag with an affinity constant of 0.286 nM as determined in a SPR assay (Biacore T200).
Biotinylated Human PD-1, Fc,Avitag at 1 ug/mL (5 uL/well) can bind Human PD-L1, His Tag with a linear range of 0.02-0.625 ug/mL as determined in a alphaLISA homogeneous assay.
