Recombinant Human CD274 protein, C-His Tag

Product Description

Cat

IMP-241

Official Symbol

CD274

Product Overview

Recombinant Human CD274 protein(AA Phe 19-Arg 238)(Accession # NP_054862.1) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.0 kDa. The protein migrates as 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Phe 19-Arg 238

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>98% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

SEC-MALS 1

The purity of Human PD-L1, His Tag was more than 90% and the molecular weight of this protein is around 35-50 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Human PD-L1, His Tag at 1 ug/mL (100 uL/well) can bind Anti-Human PD-L1 MAb (Human IgG1) with a linear range of 0.1-3 ng/mL.

Bioactivity-ELISA 2

Immobilized Human PD-L1, His Tag at series of concentration on Anti-His Tag (C-term) Antibody precoated (0.1 ug/well) plate, can bind Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) at 5 ug/mL (100 uL/well) with a linear range of 10-78 ng/mL.

Bioactivity-BLI 1

Loaded Human PD-1, Fc Tag, low endotoxin on ProteinA Biosensor, can bind Human PD-L1, His Tag with an affinity constant of 5.3 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Anti-Human PD-L1 MAb (Human IgG1) on AHC Biosensor, can bind Human PD-L1, His Tag with an affinity constant of 1.61 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-SPR 1

Human PD-1, Fc Tag captured on CM5 chip via anti-human IgG Fc antibody, can bind Human PD-L1, His Tag with an affinity constant of 3.6 uM as determined in a SPR assay (Biacore T200).

Bioactivity-SPR 2

Anti-Human PD-L1 Mab (Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human PD-L1, His Tag with an affinity constant of 0.286 nM as determined in a SPR assay (Biacore T200).

Bioactivity-alpha 1

Biotinylated Human PD-1, Fc,Avitag at 1 ug/mL (5 uL/well) can bind Human PD-L1, His Tag with a linear range of 0.02-0.625 ug/mL as determined in a alphaLISA homogeneous assay.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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