Recombinant Human CD40LG protein, N-hFc-Flag Tag

Product Description

Cat

IMP-6020

Official Symbol

CD40LG

Product Overview

Recombinant Human CD40LG protein(P29965)(13-261aa), fused with N-terminal hFc-Flag tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

N-hFc-Flag Tag

Form

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Molecular Mass

44.5 kDa

Protein length

13-261aa

AA Sequence

MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Purity

Greater than 87% as determined by SDS-PAGE.

Biological Activity

①Measured by its binding ability in a functional ELISA. Immobilized CD40L at 2 μg/ml can bind Anti- CD40L Rabbit Monoclonal Antibody（CSB-RA156386A0HU）, the EC50 is 2.353-3.232 ng/ml.

Storage

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized CD40L at 2 ug/ml can bind Anti- CD40L Rabbit Monoclonal Antibody, the EC50 is 2.353-3.232 ng/ml

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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