Recombinant Human CD40LG protein, N-hFc-Flag Tag

Product Description

Cat

IMP-6020

Official Symbol

CD40LG

Product Overview

Recombinant Human CD40LG protein(P29965)(13-261aa), fused with N-terminal hFc-Flag tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

N-hFc-Flag Tag

Form

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Molecular Mass

44.5 kDa

Protein length

13-261aa

AA Sequence

MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Purity

Greater than 87% as determined by SDS-PAGE.

Biological Activity

①Measured by its binding ability in a functional ELISA. Immobilized CD40L at 2 μg/ml can bind Anti- CD40L Rabbit Monoclonal Antibody（CSB-RA156386A0HU）, the EC50 is 2.353-3.232 ng/ml.

Storage

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized CD40L at 2 ug/ml can bind Anti- CD40L Rabbit Monoclonal Antibody, the EC50 is 2.353-3.232 ng/ml

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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