Recombinant Human TGFA Protein, No Tag

Product Description

Cat

IMP-4969

Official Symbol

TGFA

Product Overview

Recombinant human TGFa (P01135-1) (Val40-Ala89) was expressed and purified.

Description

The miR-137 served as a tumor suppressor in non-small cell lung cancer (NSCLC) and its suppressive effect is mediated by repressing TGFA expression. TGFA gene expression was significantly higher in tumor tissues compared to adjacent normal tissue and high TGFA gene expression strongly correlated with poor survival in patients with lung adenocarcinoma, and miR-374a suppresses lung adenocarcinoma cell proliferation and invasion via targeting TGFA gene expression. Transforming growth factor alpha (TGFA) is a well-characterized mammalian growth factor that might contribute to the development of Cleft lip and palate (CL/P).

Expression System

E. coli

Species

Human

Tag

No Tag

Predicted N Terminal

Met

Form

Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant human TGFa consists of 50 amino acids and has a predicted molecular mass of 5.6 kDa. The apparent molecular mass of it is approximately 13 kDa in SDS-PAGE under reducing conditions.

Protein length

Val40-Ala89

Endotoxin

Please contact us for more information.

Purity

> 90 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2.5 ng/mL.

Bioactivity-ELISA 2

Measured by its binding ability in a functional ELISA. Immobilized human TGF-alpha at 2 μg/mL (100 μL/well) can bind Human EGFR hFc, the EC50 of Human EGFR hFc is 80-500 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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