Recombinant Human TNF protein, C-His Tag

Product Description

Cat

IMP-612

Official Symbol

TNF

Product Overview

Recombinant Human TNF protein(NP_000585.2)(Leu 25-Ser 645), fused to C-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 70.5kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 25-Ser 645

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

SEC-MALS 1

Bioactivity-ELISA 1

Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Human EGF R, His Tag with a linear range of 0.006-0.025 μg/mL.

Bioactivity-BLI 1

Loaded Erbitux (Cetuximab) on AHC Biosensor, can bind Human EGF R, His Tag with an affinity constant of 1.23 nM as determined in BLI assay.

Bioactivity-SPR 1

Erbitux (Cetuximab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human EGF R, His Tag with an affinity constant of 1.3 nM as determined in a SPR assay.

Bioactivity-FACS 1

2e5 of Anti-EGFR CAR-293 cells were stained with 100 μL of 1 μg/mL of Human EGF R, His Tag and negative control protein respectively, washed and then followed by PE anti-His antibody and analyzed with FACS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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