Recombinant Human TNF protein, C-His Tag

Product Description

Cat

IMP-612

Official Symbol

TNF

Product Overview

Recombinant Human TNF protein(NP_000585.2)(Leu 25-Ser 645), fused to C-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 70.5kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 25-Ser 645

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

SEC-MALS 1

Bioactivity-ELISA 1

Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Human EGF R, His Tag with a linear range of 0.006-0.025 μg/mL.

Bioactivity-BLI 1

Loaded Erbitux (Cetuximab) on AHC Biosensor, can bind Human EGF R, His Tag with an affinity constant of 1.23 nM as determined in BLI assay.

Bioactivity-SPR 1

Erbitux (Cetuximab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human EGF R, His Tag with an affinity constant of 1.3 nM as determined in a SPR assay.

Bioactivity-FACS 1

2e5 of Anti-EGFR CAR-293 cells were stained with 100 μL of 1 μg/mL of Human EGF R, His Tag and negative control protein respectively, washed and then followed by PE anti-His antibody and analyzed with FACS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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