Recombinant Human VCAM1 Protein (Phe25-Glu698), C-6×His-tagged
Product Description
Cat
IMP-9571
Official Symbol
VCAM1
Product Overview
Recombinant human VCAM-1/CD106 protein (Phe25-Glu698), with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.
Expression System
CHO
Species
Human
Tag
C-6×His
Predicted N Terminal
Phe25
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 75 kDa
SDS-PAGE: 80-110 kDa, under reducing conditions
Protein length
Phe25-Glu698
Bio-activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. The ED50 for this effect is 0.5-3 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
2 weeks, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. The ED50 for this effect is 0.5-3 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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