Recombinant Monkey CD22 Protein (Asp20-Arg687), C-hFc-tagged

Product Description

Cat

IMP-10271

Official Symbol

CD22

Product Overview

Recombinant Cynomolgus Siglec-2/CD22 Fc Chimera protein (Asp20-Arg687) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Human embryonic kidney cell.

Description

Predicted to enable CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in B cell activation; negative regulation of B cell receptor signaling pathway; and regulation of endocytosis. Located in early endosome and recycling endosome.

Expression System

HEK293

Species

Monkey

Tag

C-hFc

Predicted N Terminal

Asp20

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Molecular Mass

Predicted Molecular Mass: 102 kDa
SDS-PAGE: 115-134 kDa, reducing conditions

Protein length

Asp20-Arg687

Bio-activity

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is 0.07-0.42 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 250 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is 0.07-0.42 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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