Recombinant Monkey CD22 Protein (Asp20-Arg687), C-hFc-tagged

Product Description

Cat

IMP-10271

Official Symbol

CD22

Product Overview

Recombinant Cynomolgus Siglec-2/CD22 Fc Chimera protein (Asp20-Arg687) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Human embryonic kidney cell.

Description

Predicted to enable CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in B cell activation; negative regulation of B cell receptor signaling pathway; and regulation of endocytosis. Located in early endosome and recycling endosome.

Expression System

HEK293

Species

Monkey

Tag

C-hFc

Predicted N Terminal

Asp20

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Molecular Mass

Predicted Molecular Mass: 102 kDa
SDS-PAGE: 115-134 kDa, reducing conditions

Protein length

Asp20-Arg687

Bio-activity

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is 0.07-0.42 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 250 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is 0.07-0.42 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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